Confocal immunofluorescent analysis of rat cerebellum using S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 647 Conjugate) #5548 (blue pseudocolor) and β3-Tubulin (D71G9) XP® Rabbit mAb #5568 (green). Red = Propidium Iodide (PI)/RNase Staining Solution.
Confocal immunofluorescent analysis of HeLa cells using α-Tubulin (DM1A) Mouse mAb #3873 (green). Red = Propidium Iodide (PI)/RNase Staining Solution.
Flow cytometric analysis of untreated Jurkat cells, using Phospho-Histone H3 (Ser10) Antibody (Alexa Fluor® 488 Conjugate) #9708 and Propidium Iodide (PI)/RNase Staining Solution (DNA content).
As a last step for fluorescent assays, add enough neat (undiluted) PI/RNase Staining Solution to submerge cells or samples. Incubate for 15 minutes at room temperature, protected from light before analysis.
Supplied in aqueous buffered solution containing less than 0.09% sodium azide. As with all intercalating DNA dyes, take all necessary safety precautions when using.
Store at 4°C protected from light, do not aliquot or dilute. The solution is stable for 12 months.Concentration:PI = 10ug/mlRNase = 300 ug/ml
Propidium Iodide (PI) is a fluorescent dye which intercalates between bases and stains both DNA and RNA. Specific DNA staining is achieved by enzymatic removal of RNA with a ribonuclease (RNase). PI/RNase is commonly used as a nuclear stain in fluorescent microscopy and as a DNA content determinant in cell cycle analyses by flow cytometry. Cells in G2 and M phases of the cell cycle contain twice the DNA content compared to those in G0 and G1 phases. DNA content during S phase lies between these extremes. PI/RNase Staining Solution has excitation and emission maxima of 535 and 617 nm, respectively (orange to red range of the spectrum). This staining solution can be used in tandem with antibodies without binding interference and is a suitable DNA stain for multiplex assays. (1-3)
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