Vortex tube briefly to resuspend the beads. Add 30 µl of bead slurry to each chromatin immunoprecipitation (ChIP) reaction. For bead washing and subsequent elution of immunocomplexes, the beads can be separated from solution by a brief 1 minute centrifugation in a microcentrifuge at 6,000 rpm. Resuspend the beads in solution by gently vortexing or rocking the tube.
These beads are specifically formulated for ChIP assays, if working with immunoprecipitation (IP) assays please see 37478
Supplied in 10 mM Tris-HCl (pH 7.5), 1 mM EDTA, 500 µg/ml BSA, 200 µg/ml sonicated salmon sperm DNA and 0.05% sodium azide. Store at 4°C.
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|9007S||1 ml (30 immunoprecipitations)||