For the CUT&RUN assay, we recommend adding 1 μl 100X Spermidine #27287, 0.5 μl Protease Inhibitor Cocktail (200X) #7012, and 2.5 μl Digitonin Solution #16359 to 96 μl CUT&RUN Antibody Binding Buffer (100 μl per reaction) right before use. Please keep on ice during use. The volume of Digitonin Solution #16359 in the CUT&RUN Antibody Binding Buffer can be adjusted based on the specific cell line.
Store CUT&RUN Antibody Binding Buffer at 4°C. This product is stable for at least 12 months.
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