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42953
Human Amphiregulin Recombinant Protein
Cytokines & Growth Factors
Growth Factors and Cytokines

Human Amphiregulin Recombinant Protein #42953

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Human Amphiregulin Recombinant Protein: Image 1
Serial dilutions of Human Amphiregulin Recombinant Protein were added to 3T3 cells. Cell proliferation was measured and the linear portion of the curve was used to calculate the ED50.
Human Amphiregulin Recombinant Protein: Image 2
The purity of Human Amphiregulin Recombinant Protein was determined by SDS-PAGE of 1 µg reduced (+) and non-reduced (-) recombinant hAmphiregulin and staining with Coomassie Blue.

Storage

Human Amphiregulin Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/mL which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.

Product Description

MW (kDa) 10.1
Purity A greater than or equal to 95% purity was determined by SDS-PAGE.
Endotoxin Endotoxin levels are less than or equal to 1 EU / 1 μg hAmphiregulin.
Activity The bioactivity of recombinant hAmphiregulin was determined in a 3T3 cell proliferation assay. The ED50 of each lot is less than or equal to 20 ng/mL.

Source / Purification

Recombinant human Amphiregulin was expressed in E. coli and is supplied in a lyophilized form.

Background

Amphiregulin (AREG) is essential for mammary ductal development and is expressed in many parts of the body, such as the ovary, placenta, pancreas, breast, lung, and spleen (1,2). Studies on preneoplastic human breast tissue and breast cancer cell lines suggest a significant role for amphiregulin in driving human breast cancer progression (1). Amphiregulin interacts with the epidermal growth factor (EGF) receptor to promote the growth of normal epithelial cells (3). It drives the proliferation of fibroblasts and production of cytokines interleukin 8 (IL-8) and vascular endothelial growth factor (VEGF) which suggests a proinflammatory role as observed in rheumatoid arthritis patients (4).
  1. McBryan, J. et al. (2008) J Mammary Gland Biol Neoplasia 13, 159-69.
  2. Busser, B. et al. (2011) Biochim Biophys Acta 1816, 119-31.
  3. Shoyab, M. et al. (1989) Science 243, 1074-6.
  4. Yamane, S. et al. (2008) J Inflamm (Lond) 5, 5.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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To Purchase # 42953
Cat. # Size Qty. Price
42953S
10 µg
$ 95