Human GDF5 Recombinant Protein #72306
Product Information
Storage
Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Product Description
| MW (kDa) | 27.4 |
| Purity | A greater than or equal to 95% purity was determined by SDS-PAGE. |
| Endotoxin | Endotoxin levels are less than or equal to 1 EU / 1 μg hGDF5. |
| Activity | The bioactivity of recombinant hGDF5 was determined by measuring the alkaline phosphatase activity in ATDC-5 cells. The ED50 of each lot is less than or equal to 1.2 μg/mL. |
Source / Purification
Recombinant human GDF5 was expressed in E. coli and is supplied in a lyophilized form.
Background
Bone morphogenetic proteins (BMPs), also known as growth differentiation factors (GDFs), were first identified as molecules that can induce ectopic bone and cartilage formation (1,2). BMPs are synthesized as precursor proteins that are processed by cleavage to produce mature proteins. BMPs initiate signaling by binding to a receptor complex containing type I and type II serine/threonine receptor kinases that then phosphorylate Smad (mainly Smad1, 5, and 8), resulting in the translocation of Smad to the nucleus. BMP was also reported to activate MAPK pathways in some systems (3,4).
Growth differentiation factor 5 (GDF5), also known as BMP-14, belongs to the BMP family of transforming growth factor beta (TGF-β) superfamily (5). GDF plays a critical role in skeletal, limb, and joint development (6,7). Decreased or altered expression of GDF5 leads to the pathogenesis of osteoarthritis, as well as Parkinson's disease (7-9).
- Wang, E.A. et al. (1988) Proc Natl Acad Sci U S A 85, 9484-8.
- Wozney, J.M. et al. (1988) Science 242, 1528-34.
- Kawabata, M. et al. (1998) Cytokine Growth Factor Rev 9, 49-61.
- Nohe, A. et al. (2004) Cell Signal 16, 291-9.
- Chang, S.C. et al. (1994) J Biol Chem 269, 28227-34.
- Buxton, P. et al. (2001) J Bone Joint Surg Am 83-A Suppl 1, S23-30.
- Degenkolbe, E. et al. (2013) PLoS Genet 9, e1003846.
- Zhang, Y. et al. (2014) Exp Mol Med 46, e79.
- Sullivan, A.M. and O'Keeffe, G.W. (2005) J Anat 207, 219-26.
Limited Uses
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