Human IL-33 Recombinant Protein #61135
Product Information
Storage
Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Product Description
| MW (kDa) | 18 |
| Purity | A greater than or equal to 95% purity was determined by SDS-PAGE. |
| Endotoxin | Endotoxin levels are less than or equal to 1 EU / 1 μg hIL-33. |
| Activity | The bioactivity of recombinant hIL-33 was determined in a D10S cell proliferation assay. The ED50 of each lot is less than or equal to 500 pg/ml. |
Source / Purification
Recombinant human IL-33 was expressed in E. coli and is supplied in a lyophilized form.
Background
IL-33 (Interleukin-33, NF-HEV) is a nuclear cytokine that is part of the IL-1 family (1,2). Originally discovered as the ‘nuclear factor from high endothelial venules,’ it is widely expressed by endothelial cells as well as epithelial barrier tissues (1,5). IL-33 is involved in regulating type 2-associated innate immune responses (2,4). It is the ligand for ST2 which, along with IL-1RAcP, forms the IL-33R signaling complex (2,3). IL-33 functions as an alarmin upon cellular damage or stress, and is associated with allergic inflammation and asthma (5,6).
- Baekkevold, E.S. et al. (2003) Am J Pathol 163, 69-79.
- Schmitz, J. et al. (2005) Immunity 23, 479-90.
- Chackerian, A.A. et al. (2007) J Immunol 179, 2551-5.
- Smithgall, M.D. et al. (2008) Int Immunol 20, 1019-30.
- Moussion, C. et al. (2008) PLoS One 3, e3331.
- Cayrol, C. and Girard, J.P. (2014) Curr Opin Immunol 31, 31-7.
Limited Uses
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