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Human Interleukin-5 (hIL-5)

Human Interleukin-5 (hIL-5) #5924

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Product Information

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-5. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Storage

Stable in lyophilized state at 4°C for >1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for >2 months and at -20°C for >6 months. Avoid repeated freeze-thaw cycles.
Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

Product Description

MW (kDa)	14-21
Purity	>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-5. All lots are greater than 98% pure
Endotoxin	Less than 0.01 ng endotoxin/1 μg hIL-5.
Activity	The activity of hIL-5 was assessed by quantification of phospho-p44/42 MAPK (Erk1/2) in butyric acid differentiated HL-60 Clone 15 cells. The observed ED₅₀ values are between 0.50-1 ng/ml.
Molecular Formula	Recombinant hIL-5 contains no "tags" and the nonglycosylated protein has a calculated MW of 13,149. DTT-reduced protein migrates as a 14-21 kDa polypeptide. Heterogeneity and lower mobility in SDS-PAGE is due to glycosylation. The non-reduced cystine-linked homodimer migrates as a 32-38 kDa protein. The expected amino-terminal IPTEI of recombinant hIL-5 was verified by amino acid sequencing.

Source / Purification

Recombinant human IL-5 (hIL-5) Ile20-Ser134 (Accession #NP_000870) was expressed in human 293 cells at Cell Signaling Technology.

Background

IL-5 is a pleiotrophic cytokine that is predominantly produced by TH2 T cells but can also be expressed by activated eosinophils, mast cells, NK cells, and iNKT cells (1-4). Both human and mouse IL-5 are glycosylated disulfide-linked homodimers (1). The IL-5 receptor is a heterodimer that consists of a high affinity IL-5 binding α chain and the common β chain, which is shared by GM-CSF and IL-3 receptors, for signal transduction (1,2). Soluble IL-5Rα binds with high affinity to IL-5, thereby inhibiting IL-5 activity (1). IL-5-mediated signaling can activate the Erk1/2, Jak2, and Stat5 signaling pathways (1,2). In mice, IL-5 is important for the differentiation of antibody secreting cells from activated B cells (2). IL-5 induces eosinophil activation, proliferation, and differentiation in both mice and humans (2,3).

Database Links

UniProt ID: P05113

Entrez-Gene Id: 3567

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