Human Transforming Growth Factor β2 (hTGF-β2) #8406
Inquiry Info. # 8406
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Product Information
Product Usage Information
Reconstitution:
With carrier: Add sterile 4 mM HCl containing 0.1% BSA to a final hTGF-β2 concentration of greater than 50 μg/ml. Solubilize for 30 minutes at room temperature with occasional gentle vortexing.
Carrier free: Add sterile 4 mM HCl, or 4 mM HCl containing protein to minimize absorption of hTGF-β2 to surfaces. Solubilize for 30 minutes at room temperature with occasional gentle vortexing. Stock hTGF-β2 should be greater than 50 μg/ml.
Formulation
Storage
Maintain sterility. Storage at -20°C should be in a manual defrost freezer.
Product Description
| MW (kDa) | 12 |
| Purity | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hTGF-β2. All lots are greater than 98% pure. |
| Endotoxin | Less than 0.01 ng endotoxin/1 μg hTGF-β2. |
| Activity | The bioactivity of recombinant hTGF-β2 was was determined by assessing inhibition of IL-4 induced HT-2 cell proliferation. The ED50 of each lot is between 0.1-0.3 ng/ml. |
| Molecular Formula | Recombinant hTGF-β2 contains no "tags" and the nonglycosylated protein has a calculated MW of 12,719. DTT-reduced protein migrates as a 12 kDa polypeptide and the non-reduced cystine-linked homodimer migrates as a 24 kDa protein. The expected amino-terminal ALDAA of recombinant hTGF-β2 was verified by amino acid sequencing. |
Source / Purification
Background
- Balzar, S. et al. (2005) J Allergy Clin Immunol 115, 110-7.
- Siegel, P.M. and Massagué, J. (2003) Nat Rev Cancer 3, 807-21.
- Bierie, B. and Moses, H.L. (2006) Nat Rev Cancer 6, 506-20.
- Hinz, S. et al. (2007) Cancer Res 67, 8344-50.
- Damstrup, L. et al. (1993) Br J Cancer 67, 1015-21.
- Constam, D.B. et al. (1992) J Immunol 148, 1404-10.
- Zhang, H. et al. (2008) Immunology 124, 304-14.
- Dufour, C. et al. (2008) Am J Physiol Endocrinol Metab 294, E794-801.
- Moustakas, A. and Heldin, C.H. (2009) Development 136, 3699-714.
- Dünker, N. and Krieglstein, K. (2000) Eur J Biochem 267, 6982-8.
Alternate Names
TGF beta-2; TGFb2
Limited Uses
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