Mouse IL-22 Recombinant Protein #58269
Product Information
Storage
Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Product Description
| MW (kDa) | 16.8 |
| Purity | A greater than or equal to 95% purity was determined by SDS-PAGE. |
| Endotoxin | Endotoxin levels are less than or equal to 1 EU / 1 μg mIL-22. |
| Activity | The bioactivity of recombinant mIL-22 was determined by measuring the production of IL-10 from human Colo 205 cells. The ED50 of each lot is less than or equal to 1 ng/ml. |
Source / Purification
Recombinant mouse IL-22 was expressed in E. coli and is supplied in a lyophilized form.
Background
Interleukin-22 (IL-22), a member of the IL-10 family (1,2), is expressed by Th17 CD4+ T cells, activated T cells, Th1 cells, and NK cells (3). Expression of IL-22 in combination with a select group of cytokines defines a subset of Th cells (4). IL-22 induces proinflammatory responses, drives production of antimicrobial peptides, and is involved in tissue repair and wound healing responses (1). The IL-22 receptor is a heterodimer of IL-22R1 and IL-10R2 (5). Expression of IL-22R is restricted to tissue-resident cells, particularly those of epithelial origin, whereas the IL-10R2 chain is expressed in many more cell types. IL-22 induces phosphorylation of Jak1 and Tyk2, leading to activation of Stat3 and, to a lesser extent, Stat1 and Stat5 (1). IL-22 responses can involve activation of the MEK-ERK-RSK, JNK-SAPK, and p38 pathways (1). Elevated levels of IL-22 have been associated with Crohn's disease and rheumatoid arthritis. IL-22 plays an essential role in host response to the pulmonary pathogen Klebsiella pneumoniae (6).
- Ouyang, W. et al. (2008) Immunity 28, 454-67.
- Dumoutier, L. et al. (2000) Proc Natl Acad Sci U S A 97, 10144-9.
- Takatori, H. et al. (2008) Mod Rheumatol 18, 533-41.
- Eyerich, S. et al. (2009) J Clin Invest 119, 3573-85.
- Nagalakshmi, M.L. et al. (2004) Int Immunopharmacol 4, 679-91.
- Aujla, S.J. et al. (2008) Nat Med 14, 275-81.
Limited Uses
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