Mouse Interleukin-17A (mIL-17A) #5227
Inquiry Info. # 5227
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Product Information
Formulation
Storage
Maintain sterility. Storage at -20°C should be in a manual defrost freezer.
Product Description
| MW (kDa) | 15-22 |
| Purity | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-17A. All lots are greater than 98% pure. |
| Endotoxin | Less than 0.01 ng endotoxin/1μg mIL-17A. |
| Activity | The bioactivity of recombinant mIL-17A was determined by its ability to induce mouse IL-6 production by 3T3 MEFs WT. The ED50 of each lot is between 0.4-1.4 ng/ml. |
| Molecular Formula | Recombinant mIL-17A contains no "tags" and the nonglycosylated protein has a calculated MW of 15,377. DTT-reduced protein migrates as a 15-22 kDa polypeptide. Heterogeneity in SDS PAGE is due to glycosylation. The non-reduced cystine-linked homodimer migrates as a 28-36 kDa protein. The expected amino-terminal TVKAA of recombinant mIL-17A was verified by amino acid sequencing. |
Source / Purification
Background
- Kolls, J.K. and Lindén, A. (2004) Immunity 21, 467-76.
- Liang, S.C. et al. (2006) J Exp Med 203, 2271-9.
- Dubin, P.J. and Kolls, J.K. (2008) Immunol Rev 226, 160-71.
- Zrioual, S. et al. (2009) J Immunol 182, 3112-20.
- Wright, J.F. et al. (2008) J Immunol 181, 2799-805.
- Rahman, M.S. et al. (2006) J Immunol 177, 4064-71.
Alternate Names
Il-17; il17a
Limited Uses
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