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8910
Human Basic Fibroblast Growth Factor (hFGF basic/FGF2)

Human Basic Fibroblast Growth Factor (hFGF basic/FGF2) #8910

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Coomassie Gel Image 1

The purity of recombinant hFGF basic was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hFGF basic and staining overnight with Coomassie Blue.

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Bioactivity Image 2

The proliferation of NIH/3T3 cells treated with increasing concentrations of hFGF basic was assessed. After 24 hr treatment, cells were labeled with BrdU for 4 hrs. BrdU incorporation was determined by ELISA and the OD450-OD690 was determined.

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Western Blotting Image 3

Western blot analysis of extracts from NIH/3T3 cells, untreated or treated with Human Basic Fibroblast Growth Factor for 10 minutes, using Phospho-CREB (Ser133) Antibody #9191 (upper) and CREB Antibody #9192 (lower).

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Recombinant human FGF basic (hFGF basic) Pro143-Ser288 (Accession #NP_001997) was produced in E. coli at Cell Signaling Technology.

Purity:

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hFGF basic. All lots are greater than 98% pure.

Molecular Formula:

Based on amino acid sequencing, 60-80% of recombinant hFGF basic starts at the amino-terminal Pro143 (PALPE) and has a calculated MW of 16,539. The remainder is missing Pro143 and starts at Ala144 (ALPED). DTT-reduced and non-reduced protein migrate as 17 kDa polypeptides.

Bioactivity:

The bioactivity of recombinant hFGF basic was determined in a NIH/3T3 cell proliferation assay. The ED50 of each lot is between 0.2 - 1.0 ng/ml.

Endotoxin:

Less than 0.01 ng endotoxin/1 μg hFGF basic.

Formulation:

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 10mM DTT and 20 μg BSA per 1 μg hFGF basic. Cystines are not required for bioactivity. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 10mM DTT. Cystines are not required for bioactivity.

Storage:

Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

FGF basic (FGF2) is produced by epithelial, tumor and other cell types (1). FGF basic is involved in developmental processes and regulates differentiation, proliferation, and migration (1-6). FGF basic is a critical factor for growing embryonic stem cells in culture without inducing differentiation. FGF basic has a high affinity for heparan sulfate (1,2) and binding is a step in the FGF basic activation of FGFR tyrosine kinase. There are four distinct FGF receptors and each has multiple splice variants. FGF basic binds with high affinity to many, but not all, FGFRs. Signaling cascades activated through FGF basic binding to FGFR include the ras-raf-MAPK, PLCγ/PKC, and PI3K/AKT pathways (1).

  1. Dvorak, P. and Hampl, A. (2005) Folia Histochem Cytobiol 43, 203-8.
  2. Ornitz, D.M. and Itoh, N. (2001) Genome Biol 2, REVIEWS3005.
  3. Shi, Y. et al. (2008) Crit Rev Oncol Hematol 65, 43-53.
  4. Fontijn, D. et al. (2006) Br J Cancer 94, 1627-36.
  5. Marek, L. et al. (2009) Mol Pharmacol 75, 196-207.
  6. Acevedo, V.D. et al. (2009) Cell Cycle 8, 580-8.
Entrez-Gene Id
2247
Swiss-Prot Acc.
P09038
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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