|8927||Human Interferon-α1 (hIFN-α1)||
Immunofluorescent staining of untreated (left) and IFN-alpha (100 ng/ml for 5 minutes) treated HeLa cells, using Phospho-Stat1 (Tyr701) Antibody #9171.
Western blot analysis of lysates from HeLa cells treated with Human Interferon-alpha (IFN-alpha) (100 ng/ml for 5 minutes), using Phospho-Stat3 (Tyr705) (3E2) Monoclonal Antibody #9138 (upper) and Stat3 Antibody #9132 (lower).
Western blot analysis of lysates from murine A20 cells treated with 100 ng/ml Human Interferon-alpha (IFN-alpha) for 5 minutes, using Phospho-Stat1 (Tyr701) Antibody #9171 (upper) and Stat1 Antibody #9172 (lower).
Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles. Maintain sterility. Storage at -20°C should be in a manual defrost freezer.
Directions for Use: Human Interferon-alpha (IFN-alpha) is supplied as a solution. It should be stored at -80 degrees Centigrade. Aliquot the reagent upon receipt and avoid repeated freeze-thaw cycles.
Interferons (IFNs) appear both locally and systematically early after viral infection and participate in limiting the spread of infection. They also affect cell differentiation, growth, surface antigen expression and immunoregulation (1). There are three naturally occurring interferons: α, β and γ. IFN-α is derived from lymphoblastic tissue and has a number of therapeutic applications in the treatment of various human cancers and diseases of viral origin. Recombinant IFN-α from both natural and synthetic genes binds to a common cell surface receptor and induces antiviral activity in a variety of cell lines. When binding to discrete cell surface receptors on target cells, IFN-α induces rapid changes in Jak/Stat phosphorylation, which initiates the Jak/Stat signaling pathway (2). IFN-α signaling also involves production of DAG without an increased intracellular free calcium concentration and the subsequent activation of calcium-independent isoforms of PKC (β and ε) (3). All IFN-α signaling pathways lead to final alterations of gene expression, which mediate their pleiotropic biologic activities.
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