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Product Information
With carrier: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl and 20 μg BSA per 1 μg hPDGF-BB. Carrier free: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl.
Stable in lyophilized state at -20°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.
Maintain sterility. Storage at -20°C should be in a manual defrost freezer.
| MW (kDa) | 14 |
| Purity | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hPDGF-BB. All lots are greater than 98% pure. |
| Endotoxin | Less than 0.01 ng endotoxin/1 μg hPDGF-BB. |
| Activity | The bioactivity of recombinant hPDGF-BB was determined in a NIH/3T3 cell proliferation assay. The ED50 of each lot is between 4.0 - 15 ng/ml. |
| Molecular Formula | Recombinant hPDGF-BB does not have a Met on the amino terminus and has a calculated MW of 12,294. DTT-reduced protein migrates as a 14 kDa polypeptide and the non-reduced cystine-linked homodimer migrates as a 32 kDa protein. The expected amino-terminal SLGSL of recombinant hPDGF-BB was verified by amino acid sequencing. |
Recombinant human PDGF-BB (hPDGF-BB) Ser82-Thr190 (Accession #NP_002599) was produced in E. coli at Cell Signaling Technology.
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| Cat. # | Size | Qty. | Price |
|---|---|---|---|
| 8912SC | 10 µg (With Carrier) | $ 227 | |
| 8912SF | 10 µg (Carrier Free) | $ 227 |
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