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Important Shipping Information

Due to a water main break, our U.S. warehouse is closed for the remainder of the day, Thursday, July 28. Orders placed today will ship tomorrow, Friday, July 29. We apologize for the inconvenience.

To Purchase # 5217SC

5217SC 10 µg (With Carrier) $199.00
5217SF 10 µg (Carrier Free) $199.00
5217LC 50 µg (With Carrier) $599.00
5217LF 50 µg (Carrier Free) $599.00
$0.00

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Source / Purification

Recombinant mouse IL-7 (mIL-7) Glu26-Ile154 (Accession #NP_032397) was produced in E. coli at Cell Signaling Technology.

Product Description

Purity:

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-7. All lots are greater than 98% pure.


Molecular Formula:

Recombinant mIL-7 has a Met on the amino terminus and has a calculated MW of 15,028. DTT-reduced and non-reduced protein migrate as 14 kDa polypeptides. The expected amino-terminal MECHI of recombinant mIL-7 was verified by amino acid sequencing.


Bioactivity:

The bioactivity of recombinant mIL-7 was determined in a 2E8 cell proliferation assay. The ED50 of each lot is between 60-200 pg/ml.


Endotoxin:

Less than 0.01 ng endotoxin/1 μg mIL-7.


Product Usage Information

Formulation:

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mIL-7. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.


Storage: Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

IL-7 plays a key role in lymphopoiesis and lymphoid homeostasis (1). Stromal and epithelial cells within the bone marrow and thymus produce IL-7 (1). The primary targets of IL-7 are T cells, B cells, and dendritic cells (1). IL-7 is crucial for T cell development, the importance of which is underscored by the lack of T cells in both mice and humans that are deficient in IL-7/IL-7R signaling (1,2). While IL-7 appears to be required for B cell development in mice, the role of IL-7 in human B cell development is unclear (1,3). In addition to its effects on T cell lymphopoiesis, IL-7 promotes the maintenance and survival of naïve and memory αβ T cells, as well as γδ T cells (1). The IL-7 receptor is a heterodimer of the common γ chain, γc, and the IL-7-specific IL-7Rα (1). IL-7 activates PI3K/Akt, Jak1/2, and Stat1, 3, and 5 (1).


1.  Rochman, Y. et al. (2009) Nat Rev Immunol 9, 480-90.

2.  Ma, A. et al. (2006) Annu Rev Immunol 24, 657-79.

3.  Parrish, Y.K. et al. (2009) J Immunol 182, 4255-66.


Entrez-Gene Id 16196
Swiss-Prot Acc. P10168


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

5217
Mouse Interleukin-7 (mIL-7)