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5228
Mouse Macrophage Colony Stimulating Factor (mM-CSF)

Mouse Macrophage Colony Stimulating Factor (mM-CSF) #5228

Coomassie Gel Image 1

The purity of recombinant mM-CSF was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mM-CSF and staining overnight with Coomassie Blue.

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Bioactivity Image 2

The proliferation of M-NFS-60 cells treated with increasing concentrations of mM-CSF was assessed. After 48 hour treatment with mM-CSF, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.

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Western Blotting Image 3

Western blot analysis of extracts from M-NFS-60 cells, untreated or treated with mM-CSF for 10 minutes, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (upper) and p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (lower).

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Recombinant mouse M-CSF (mM-CSF) Lys33-Glu262 (Accession #NP_031804) was expressed in human 293 cells at Cell Signaling Technology.

Purity:

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mM-CSF. All lots are greater than 98% pure.

Molecular Formula:

Recombinant mM-CSF contains no "tags" and the nonglycosylated protein has a calculated MW of 25,987. DTT-reduced protein migrates as a 37-50 kDa polypeptide and the non-reduced cystine-linked homodimer migrates as a 55-80 kDa protein. Heterogeneity and lower mobility in SDS-PAGE are due to glycosylation. The expected amino-terminal KEVSE of recombinant mM-CSF was verified by amino acid sequencing.

Bioactivity:

The bioactivity of recombinant mM-CSF was determined in an M-NFS-60 cell proliferation assay. The ED50 of each lot is between 0.5-2.0 ng/ml.

Endotoxin:

Less than 0.01 ng endotoxin/1 μg mM-CSF.

Formulation:

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mM-CSF. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Storage:

Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

Macrophage-colony stimulating factor (M-CSF) is produced by fibroblasts, endothelial cells, stromal cells, macrophages, osteoblasts, and other cell types (1). M-CSF is required for growth and differentiation of monocytes and macrophages (1,2). M-CSF polarizes macrophages into the M2 phenotype where anti-inflammatory IL-10 is produced, rather than the M1 phenotype where inflammatory cytokines are produced. M-CSF also recruits monocytes and enhances angiogenesis by inducing VEGF production (1,2). M-CSF binds to its receptor CSF1R; downstream signaling involves PI3K/Akt, Erk, and Stats 1, 3, and 5 (1,3). An increase in M-CSF expression may contribute to cancer progression, and high plasma M-CSF levels are associated with rheumatoid arthritis (1,4,5).

  1. Hamilton, J.A. (2008) Nat Rev Immunol 8, 533-44.
  2. Curry, J.M. et al. (2008) PLoS One 3, e3405.
  3. Rioja, I. et al. (2008) Arthritis Rheum 58, 2257-67.
  4. Skrzypski, M. et al. (2008) Clin Cancer Res 14, 4794-9.
  5. Hamilton, J.A. (1997) J Leukoc Biol 62, 145-55.
Entrez-Gene Id
12977
Swiss-Prot Acc.
P07141
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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To Purchase # 5228SC

Product Number Size Price
5228SC 10 µg (With Carrier) $204.00.0
5228SF 10 µg (Carrier Free) $204.00.0
Quantity Subtotal
$0.00