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5211
Mouse Vascular Endothelial Growth Factor-164 (mVEGF164 )
Cytokines
Growth Factors and Cytokines

Mouse Vascular Endothelial Growth Factor-164 (mVEGF164 ) #5211

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other Image 1 - Mouse Vascular Endothelial Growth Factor-164 (mVEGF164 )

The proliferation of HUVEC treated with increasing concentrations of mVEGF164 was assessed. After 72-hour treatment with mVEGF164 cells were incubated with a tetrazolium salt and the OD450-OD650 was determined.

other Image 2 - Mouse Vascular Endothelial Growth Factor-164 (mVEGF164 )

The purity of recombinant mVEGF164 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mVEGF164 and staining overnight with Coomassie Blue.

other Image 3 - Mouse Vascular Endothelial Growth Factor-164 (mVEGF164 )

Western blot analysis of extracts from HUVEC untreated or treated with mVEGF164 for 15 minutes, using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 (upper) and Akt1 (C73H10) Rabbit mAb #2938 (lower).

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mVEGF164. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Storage

Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

Product Description

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mVEGF164. All lots are greater than 98% pure.

Molecular Formula

Recombinant mVEGF164 contains no "tags" and the nonglycosylated protein has a calculated MW of 19,278. DTT-reduced protein migrates as a 24-31 kDa polypeptide. Lower mobility in SDS-PAGE is due to glycosylation. The non-reduced cystine-linked homodimer migrates as a 38-44 kDa protein. The expected amino-terminal APTTE of recombinant mVEGF164 was verified by amino acid sequencing.

Source / Purification

Recombinant mouse VEGF164 (mVEGF164) Ala205-Arg368 (Accession #NP_033531) was expressed in human 293 cells at Cell Signaling Technology.

Background

VEGF164 is one of many splice variants of the mouse VEGF-A gene, and is one amino acid shorter than its human counterpart, VEGF165 (1,2). VEGF164 is produced by a number of cells including endothelial cells, macrophages and T cells (1,2). VEGF164 is involved in angiogenesis, vascular endothelial cell survival, growth, migration and vascular permeability (1,2). Gene expression is induced by hypoxia, inflammatory cytokines and oncogenes (1,2). VEGF164 binds to heparan sulfate and is retained on the cell surface and in the extracellular matrix (1-3). VEGFR1 and VEGFR2 are the receptor tyrosine kinases for VEGF164 (2). NRP-1 and NRP-2 may function as co-receptors and enhance VEGFR2 signaling (2-3). Binding of VEGF164 to VEGFR1 and VEGR2 leads to activation of the PI3K/AKT, p38 MAPK, FAK and Paxillin (2).

  1. Haigh, J.J. (2008) Organogenesis 4, 247-56.
  2. Takahashi, H. and Shibuya, M. (2005) Clin Sci (Lond) 109, 227-41.
  3. Neufeld, G. et al. (1999) FASEB J 13, 9-22.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 5211SC
Product # Size Price
5211SC
10 µg  (With Carrier) $ 221
5211SF
10 µg  (Carrier Free) $ 221