SignalKine™ Human EGF Chemiluminescent Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects human EGF (hEGF) in multiple matrices. Unknown samples being tested for hEGF and hEGF standards are added to low volume microwells, where the hEGF is captured by the coated hEGF Rabbit mAb. Following a washing step, a biotinylated hEGF Detection Rabbit mAb is added to detect the captured hEGF. HRP-linked Streptavidin is then used for detection of the biotinylated hEGF Detection Rabbit mAb. A chemiluminescent reagent is added for signal development. The magnitude of light emission, measured in relative light units (RLU) is proportional to the quantity of human EGF in the sample.
SignalKine™ Human EGF Chemiluminescent Sandwich ELISA Kit detects hEGF in multiple matrices that can be quantified by generating a standard curve with the recombinant hEGF protein standard provided. The hEGF standard range is from 0.6 to 2500 pg/ml. Samples containing higher levels of hEGF can be diluted to fit into the standards range.
Epidermal growth factor (EGF) is a small polypeptide hormone that has mitogenic properties in vivo and in vitro, modifying the growth and/or differentiation of many cell types. EGF elicits biologic responses by binding to its cell surface receptor, a transmembrane glycoprotein containing a cytoplasmic protein tyrosine kinase domain (1,2). The binding of EGF to the EGF receptor promotes dimerization of the receptor, autophosphorylation, and activation of downstream signaling components (3). The integrated biological responses to EGF signaling are pleiotropic, including mitogenesis, apoptosis, enhanced cell motility, protein secretion, differentiation, and dedifferentiation. In addition, EGF has been implicated in organ morphogenesis, maintenance, and repair. Moreover, activation of EGF receptor signaling has been correlated with progression to invasion and metastisis in a wide variety of tumors (4-6). Research studies have identified EGF receptor and its downstream signaling molecules as potential targets for therapeutic interventions in wound repair and cancer (4-6).
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