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SignalKine Human IL-8 Chemiluminescent Sandwich ELISA Kit #7921

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    Product Specifications

    REACTIVITY H
    Application Key:
    • ELISA-ELISA 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Description

    SignalKine™ Human IL-8 Chemiluminescent Sandwich ELISA Kit from Cell Signaling Technology (CST) is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects human IL-8 (hIL-8) in multiple matrices. Unknown samples being tested for hIL-8 and hIL-8 standards are added to low volume microwells, where the hIL-8 is captured by the coated hIL-8 Rabbit mAb. Following a washing step, a biotinylated hIL-8 Detection Rabbit mAb is added to detect the captured hIL-8. HRP-linked Streptavidin is then used for detection of the biotinylated hIL-8 Detection Rabbit mAb. A chemiluminescent reagent is added for signal development. The magnitude of light emission, measured in relative light units (RLU) is proportional to the quantity of human hIL-8 in the sample.

    SignalKine™ Human IL-8 Chemiluminescent Sandwich ELISA Kit detects hIL-8 in multiple matrices that can be quantified by generating a standard curve with the recombinant hIL-8 protein standard provided. The hIL-8 standard range is from 13.7 to 10,000 pg/ml. Samples containing higher levels of hIL-8 can be diluted to fit into the standards range.

    Specificity / Sensitivity


    Species Reactivity:

    Human

    Background

    IL-8 is the prototypical CXC chemokine. It is best known for effects on neutrophils, specifically its ability to act as a chemoattractant and activate degranulation and respiratory burst (1-3). IL-8 is produced by a number of cell types, including monocytes, T cells, neutrophils, fibroblasts, endothelial cells, and others (1). In addition to its effects on neutrophils, IL-8 promotes angiogenesis, inhibits endothelial cell apoptosis, and promotes the proliferation of melanoma cells in an autocrine fashion (1). As a result, IL-8 may be a contributing factor to the development of certain cancers (1,2). There are two distinct G protein-coupled receptors for IL-8, CXCR1 and CXCR2 (1). Ligand binding induces Ca2+ mobilization and activates the PI3K, PKC, Rho, and Rac pathways (1,3).

    Alternate Names

    (Ala-IL-8)77; (Ser-IL-8)72; alveolar macrophage chemotactic factor I; beta endothelial cell-derived neutrophil activating peptide; beta-thromboglobulin-like protein; C-X-C motif chemokine 8; C-X-C motif chemokine ligand 8; Chemokine (C-X-C motif) ligand 8; CXCL8; emoctakin; GCP-1; GCP/IL-8 protein I; GCP/IL-8 protein II; GCP/IL-8 protein III; GCP/IL-8 protein IV; GCP/IL-8 protein V; GCP/IL-8 protein VI; GCP1; granulocyte chemotactic protein 1; IL-8; IL-8(1-77); IL-8(5-77); IL-8(6-77); IL-8(7-77); IL-8(8-77); IL-8(9-77); IL8; IL8/NAP1 form I; IL8/NAP1 form II; IL8/NAP1 form III; IL8/NAP1 form IV; IL8/NAP1 form V; IL8/NAP1 form VI; interleukin 8; Interleukin-8; LECT; LUCT; lung giant cell carcinoma-derived chemotactic protein; lymphocyte derived neutrophil activating peptide; lymphocyte-derived neutrophil-activating factor; LYNAP; MDNCF; MDNCF-a; MDNCF-b; MDNCF-c; MONAP; monocyte-derived neutrophil chemotactic factor; monocyte-derived neutrophil-activating peptide; NAF; NAP-1; NAP1; Neutrophil-activating factor; neutrophil-activating peptide 1; Neutrophil-activating protein 1; Protein 3-10C; SCYB8; small inducible cytokine subfamily B, member 8; T cell chemotactic factor; T-cell chemotactic factor; tumor necrosis factor-induced gene 1

    For Research Use Only. Not for Use in Diagnostic Procedures.
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