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  3. PathScan® Phospho-ATF-2 (Thr71) Sandwich ELISA kit

PathScan® Phospho-ATF-2 (Thr71) Sandwich ELISA kit #7185

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Inquiry Info. # 7185

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    Product Specifications

    REACTIVITY H M
    Application Key:
    • ELISA-ELISA 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Description

    CST's PathScan® Phospho-ATF-2 (Thr71) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of phospho-ATF-2 (Thr71) protein. A Phospho-ATF-2 (Thr 71) Antibody has been coated onto the microwells. After incubation with cell lysates, phospho-ATF-2 protein is captured by the coated antibody. Following extensive washing, a total ATF-2 Mouse mAb is added to detect the captured phospho-ATF-2 protein. HRP-linked Anti-mouse Antibody #7076 is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of optical density for this developed color is proportional to the quantity of phospho-ATF-2 (Thr71).
    Antibodies in kit are custom formulations specific to kit.

    Protocol

    Specificity / Sensitivity

    PathScan® Phospho-ATF-2 (Thr71) Sandwich ELISA kit detects endogenous levels of Phospho-ATF-2 protein. Using this Sandwich ELISA Kit #7185, a significant induction of phospho-ATF-2 in NIH/3T3 cells treated with anisomycin can be detected. However, the level of total ATF-2 (phospho- and nonphospho-), detected by 7195#7195, remains unchanged (Figure 1). This kit can also be used to detect phosphorylated ATF-2 protein in human HeLa and 293 cells. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

    Species Reactivity:

    Human, Mouse

    Background

    The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways (2-4). Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2-4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4). In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2).

    Alternate Names

    Activating transcription factor 2; activating transcription factor 2 splice variant ATF2-var2; ATF-2; ATF2; cAMP response element-binding protein CRE-BP1; cAMP responsive element binding protein 2, formerly; cAMP-dependent transcription factor ATF-2; cAMP-responsive element-binding protein 2; CRE-BP1; CREB-2; CREB2; CREBP1; Cyclic AMP-dependent transcription factor ATF-2; Cyclic AMP-responsive element-binding protein 2; HB16; histone acetyltransferase ATF2; MGC111558; TREB7

    Database Links

    UniProt ID: P15336


    Entrez-Gene Id: 1386


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    For Research Use Only. Not for Use in Diagnostic Procedures.
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