CST's PathScan® Phospho-IRS-1 (Ser302) Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Phospho-IRS-1 (Ser302) Sandwich ELISA Kit #7283. Capture and detection antibodies (100X stocks) and an HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The IRS-1 Mouse Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysate is added followed by a Phospho-IRS-1 (Ser302) Rabbit Detection Antibody and an HRP-conjugated Anti-Rabbit IgG Antibody. HRP substrate (TMB) is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of IRS-1 phosphorylated at Ser302.
*Antibodies in this kit are custom formulations specific to the kit.
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