Cell Signaling Technology Logo - Extra Large
View Featured Offers >>
  1. Products /
  2. ELISA Kits /
  3. PathScan® Phospho-IRS-2 (panTyr) Sandwich ELISA Kit

PathScan® Phospho-IRS-2 (panTyr) Sandwich ELISA Kit #7860

Filter:
  • ELISA

Inquiry Info. # 7860

Please see our recommended alternatives.

    Product Specifications

    REACTIVITY M
    Application Key:
    • ELISA-ELISA 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Description

    The PathScan® Phospho-IRS-2 (panTyr) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of IRS-2 when tyrosine phosphorylated. An IRS-2 Rabbit Antibody has been coated onto the microwells. After incubation with cell lysates, IRS-2 (phospho and nonphospho) is captured by the coated antibody. Following extensive washing, a Phospho-Tyrosine Mouse Detection Antibody is added to detect tyrosine phosphorylation of the captured IRS-2 protein. Anti-mouse IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of IRS-2 phosphorylated on tyrosine.
    Antibodies in kit are custom formulations specific to kit.

    Protocol

    Specificity / Sensitivity

    PathScan® Phospho-IRS-2 (panTyr) Sandwich ELISA Kit #7860 detects IRS-2 when tyrosine phosphorylated. As shown in Figure 1, a significant induction of IRS-2 tyrosine phosphorylation can be detected in CHO (IR/4PS) cells following treatment with insulin using this Sandwich ELISA Kit #7860. The level of total IRS-2 (phospho and nonphospho) remains unchanged as shown by Western analysis. This kit also detects tyrosine phosphorylated IRS-2 in insulin treated 3T3-L1 adipocytes (data not shown). This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

    Species Reactivity:

    Mouse

    Background

    Insulin Receptor Substrate 2 (IRS-2) is one of the major substrates of the insulin receptor kinase (1). In vertebrates, IRS-2 functions as a scaffolding protein to coordinate separate branches of the Insulin/IGF-signaling cascades (2). IRS-2 is essential for normal nutrient homeostasis because it mediates both peripheral insulin action and the effect of IGF-1 on B-cell growth. Mice lacking IRS-2 fail to maintain sufficient compensatory insulin secretion and develop diabetes as young adults (3).

    Alternate Names

    Insulin receptor substrate 2; IRS-2; IRS2

    Database Links

    UniProt ID: Q9Y4H2


    Entrez-Gene Id: 8660


    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    PathScan is a registered trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.