Upstream / Downstream

pathwayImage

Explore pathways related to this product.

Important Ordering Details

Custom Ordering Details: When ordering five or more kits, please contact us for processing time and pricing at sales@cellsignal.com.

Our U.S. Offices Are Closed

Our U.S. offices are closed in observance of Memorial Day. We will reopen on Tuesday, May 30th.

Thank you for your patience.

Questions?

Find answers on our FAQs page.

ANSWERS  

PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools

LEARN MORE

REACTIVITY
Product Includes Volume Solution Color
Met Rabbit Antibody Coated Microwells 96 tests
Sealing Tape 2 sheets

Product Description

The PathScan® Phospho-Met (Tyr1349) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Met when phosphorylated at Tyr1349. A Met rabbit antibody has been coated onto the microwells. After incubation with cell lysates, phospho-Met (Tyr1349) is captured by the coated antibody. Following extensive washing, a biotinylated phospho-Met (Tyr1349) rabbit detection antibody is added to detect the captured phospho-Met protein. HRP-linked streptavidin is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The absorbance at 450nm is proportional to the quantity of Met phosphorylated at Tyr1349.

Antibodies in kit are custom formulations specific to kit.


Specificity / Sensitivity

CST's PathScan® Phospho-Met (Tyr1349) Sandwich ELISA Kit detects endogeneous levels of Met when phosphorylated at Tyr1349 (see Figure 1). The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.


Met, a high affinity tyrosine kinase receptor for hepatocyte growth factor (HGF, also known as scatter factor) is a disulfide-linked heterodimer made of 45 kDa α- and 145 kDa β-subunits (1,2). The α-subunit and the amino-terminal region of the β-subunit form the extracellular domain. The remainder of the β-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl, and PI3 kinase (3). These fundamental events are important for all of the biological functions involving Met kinase activity. The addition of a phosphate at cytoplasmic Tyr1003 is essential for Met protein ubiquitination and degradation (4). Phosphorylation at Tyr1234/1235 in the Met kinase domain is critical for kinase activation. Phosphorylation at Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (5). Research studies have shown that altered Met levels and/or tyrosine kinase activities are found in several types of tumors, including renal, colon, and breast. Thus, investigators have concluded that Met is an attractive potential cancer therapeutic and diagnostic target (6,7).


1.  Cooper, C.S. et al. (1984) Nature 311, 29-33.

2.  Bottaro, D.P. et al. (1991) Science 251, 802-4.

3.  Bardelli, A. et al. (1997) Oncogene 15, 3103-11.

4.  Taher, T.E. et al. (2002) J Immunol 169, 3793-800.

5.  Schaeper, U. et al. (2000) J Cell Biol 149, 1419-32.

6.  Eder, J.P. et al. (2009) Clin Cancer Res 15, 2207-14.

7.  Sattler, M. and Salgia, R. (2009) Update Cancer Ther 3, 109-118.


Entrez-Gene Id 4233
Swiss-Prot Acc. P08581


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a trademark of Cell Signaling Technology, Inc.

7896
PathScan® Phospho-Met (Tyr1349) Sandwich ELISA Kit