Render Target: STATIC
Render Timestamp: 2024-12-09T12:21:06.634Z
Commit: 224419269841c11382c4555dbee545259bf6c379
XML generation date: 2024-10-22 21:16:10.994
Product last modified at: 2024-10-23T07:01:07.979Z
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PDP - Template Name: ELISA Kit
PDP - Template ID: *******bd382c2

PathScan® Phospho-Stat1 (Tyr701) Sandwich ELISA Kit #73582

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  • ELISA

    Supporting Data

    REACTIVITY H M
    Application Key:
    • ELISA-ELISA 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Description

    The PathScan® Phospho-Stat1 (Tyr701) Sandwich ELISA Kit is a solid-phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Stat1 protein phosphorylated at Tyr701. A Stat1 rabbit antibody has been coated onto the microwells. After incubation with cell lysates, Stat1 protein (phosphorylated and nonphosphorylated) is captured by the coated antibody. Following extensive washing, a Phospho-Stat1 (Tyr701) mouse detection antibody is added to detect the captured phospho-Stat1 (Tyr701) protein. HRP-linked anti-mouse antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of Stat1 protein phosphorylated at Tyr701.

    *Antibodies in this kit are custom formulations specific to the kit.

    Protocol

    Specificity / Sensitivity

    The PathScan® Phospho-Stat1 (Tyr701) Sandwich ELISA Kit detects endogenous levels of Stat1 protein phosphorylated at Tyr701. The kit sensitivity is shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

    Species Reactivity:

    Human, Mouse

    Background

    The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.
    For Research Use Only. Not For Use In Diagnostic Procedures.
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