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  3. PathScan® RP Phospho-Bcl-2 (Ser70) Sandwich ELISA Kit

PathScan® RP Phospho-Bcl-2 (Ser70) Sandwich ELISA Kit #90331

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Important Ordering Details

Custom Ordering Details:

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    Product Specifications

    REACTIVITY H
    Application Key:
    • ELISA+-ELISA and/or ELISA-like Assays 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Description

    The PathScan® RP Phospho-Bcl-2 (Ser70) Sandwich ELISA Kit is a solid-phase sandwich enzyme-linked immunosorbent assay (ELISA) that recognizes endogenous levels of Phospho-Bcl-2 (Ser70) protein. A Bcl-2 Mouse Antibody has been coated on the microwells. After incubation with cell lysates, Bcl-2 proteins (phospho and nonphospho) are captured by the coated antibody. Following extensive washing, a Phospho-Bcl-2 (Ser70) Detection Antibody is added to detect captured Bcl-2 protein. Anti-rabbit IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of phospho-Bcl-2 protein.

    *Antibodies in this kit are custom formulations specific to kit.

    Protocol

    Specificity / Sensitivity

    The PathScan® RP Phospho-Bcl-2 (Ser70) Sandwich ELISA Kit detects endogenous levels of Bcl-2 protein phosphorylated at Ser70. This kit sensitivity is shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

    Species Reactivity:

    Human

    Background

    Bcl-2 exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release (1). It has been implicated in modulating mitochondrial calcium homeostasis and proton flux (2). Several phosphorylation sites have been identified within Bcl-2, including Thr56, Ser70, Thr74, and Ser87 (3). It has been suggested that these phosphorylation sites may be targets of the ASK1/MKK7/JNK1 pathway and that phosphorylation of Bcl-2 may be a marker for mitotic events (4,5). Mutation of Bcl-2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes (6). Interleukin-3 and JNK-induced Bcl-2 phosphorylation at Ser70 may be required for its enhanced anti-apoptotic functions (7).

    Alternate Names

    Apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; Bcl-2; BCL2; BCL2 apoptosis regulator; PPP1R50; protein phosphatase 1, regulatory subunit 50

    Database Links

    UniProt ID: P10415


    Entrez-Gene Id: 596


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    For Research Use Only. Not for Use in Diagnostic Procedures.
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