c-Jun Fusion Protein Beads

Inquiry Info. # 9811

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Product Specifications

MW (kDa)

35

Product Information

Product Description

c-Jun Fusion Protein Beads selectively "pull down" SAPK/JNK and also serve as a useful substrate to measure SAPK/JNK activity. It is expressed as a recombinant protein fusion to amino acid residues corresponding to c-Jun codons 1-89. The fusion protein is produced in E. coli and is supplied as a 50% slurry of c-Jun Fusion Protein beads.

MW (kDa)

35

Product Usage Information

Prior to use, put the tube on ice for 5 minutes to lower viscosity of buffer. Then beads should be resuspended to a 50% slurry by inversion or gentle vortexing.

Solutions and Reagents

Storage Buffer:20 mM Tris-HCl (pH 7.4)150 mM NaCl1 mM Na₂EDTA1 mM EGTA1% Triton X-1002.5 mM sodium pyrophosphate1 mM β-glycerophosphate1 mM Na₃VO₄1 µg/ml leupeptin50% glycerolNote: Please aliquot prior to first use.

Storage

Store at -20°C.

Background

c-Jun is a member of the Jun family containing c-Jun, JunB, and JunD, and is a component of the transcription factor activator protein-1 (AP-1). AP-1 is composed of dimers of Fos, Jun, and ATF family members and binds to and activates transcription at TRE/AP-1 elements (reviewed in 1). Extracellular signals, including growth factors, chemokines, and stress, activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knockout studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes, including axon regeneration (4), liver regeneration (5), and T cell development (6). AP-1 regulated genes exert diverse biological functions, including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9). Other target genes regulate survival, as well as hypoxia and angiogenesis (8,10). Research studies have implicated c-Jun as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, and rheumatoid arthritis (11,12).

Database Links

UniProt ID: P05412

Entrez-Gene Id: 3725

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.

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c-Jun Fusion Protein Beads #9811