Render Target: STATIC
Render Timestamp: 2024-12-02T10:54:24.844Z
Commit: cd2fae6ca3f811b1ddb1df24ac291ed56d5d501b
XML generation date: 2024-09-20 06:21:47.349
Product last modified at: 2024-11-25T13:00:30.374Z
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PDP - Template Name: Cell Extracts
PDP - Template ID: *******b5396df

Caspase-3 Control Cell Extracts #9663

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    Product Information

    Product Usage Information

    Boil for 3 minutes prior to use. Load 10 ul of untreated and cytochrome c treated Caspase-3 Control Cell Extracts per lane.

    Storage

    Supplied in SDS Sample Buffer: 62.5 mM Tris-HCl (pH 6.8 at 25°C), 2% w/v SDS, 10% glycerol,50 mM DTT, 0.01% w/v bromophenol blue or phenolred.
    Store at –20°C, or at –80°C for long-term storage.

    Product Description

    Caspase-3 Control Cell Extracts (Jurkat Untreated): Untreated Jurkat cells are lysed in Chaps cell extract buffer and a cytoplasmic fraction is generated to serve as a negative control for caspase cleavage. Supplied in SDS sample buffer.

    Caspase-3 Control Cell Extracts (Jurkat +Cytochrome c): Untreated Jurkat cells are lysed in Chaps cell extract buffer and a cytoplasmic fraction is generated. Extracts are treated with cytochrome c in vitro to generate a positive control for caspase cleavage. Supplied in SDS sample buffer.

    Background

    Caspase-3 (CPP-32, Apopain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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