News from the Bench

Discover what’s going on at CST, receive our latest application notes and tips, read our science features, and learn about our products.



Find answers on our FAQs page.


PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools


Product Includes Quantity
Cytoplasmic Isolation Buffer (CIB) 10 ml
Membrane Isolation Buffer (MIB) 10 ml
Cytoskeletal/Nuclear Isolation Buffer (CyNIB) 5 ml
Protease Inhibitor Cocktail (100X) 5871 250 µl

Product Description

The Cell Fractionation Kit is designed to provide a fast and efficient way of separating cultured cells into three distinct fractions: cytoplasmic, membrane/organelle, and nuclear/cytoskeletal. These fractions can then be analyzed by SDS-PAGE and western blotting. The kit includes enough buffer for 20 assays.

Product Usage Information

Storage: Store at -20°C. Upon receipt, Protease Inhibitor Cocktail 5871 should be stored at 4°C. All other components should be stored at -20°C.

Cellular fractionation allows for the extraction of cellular proteins into distinct compartments. This is achieved by the use of detergents that take advantage of the inherent qualities and composition of different cellular membranes (1). Cellular fractionation has been important for defining the localization of many proteins, observing the translocation of proteins, and determining protein-protein complexes, such as cytoskeletal-associated proteins (2,3). Thus, detergent-based cellular fractionation separates cellular components with greater ease and speed compared to a more laborious density centrifugation method (4).

1.  Lenstra, J.A. and Bloemendal, H. (1983) Eur J Biochem 135, 413-23.

2.  Banno, A. et al. (2012) J Biol Chem 287, 13799-812.

3.  Loo, L.H. et al. (2009) J Cell Biol 187, 375-84.

4.  Michelsen, U. and von Hagen, J. (2009) Methods Enzymol 463, 305-28.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Fractionation Kit