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1034
COX IV Blocking Peptide
Experimental Controls

COX IV Blocking Peptide #1034

This peptide is used specifically to block COX IV Antibody #4844 and COX IV (3E11) Rabbit mAb #4850 reactivity.

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks COX IV Antibody #4844 and COX IV (3E11) Rabbit mAb #4850 by dot blot.

Use as a blocking reagent to evaluate the specificity of antibody reactivity in dot blot protocols.

Storage:

Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. 1% DMSO. Store at –20°C.

Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1-3). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane to drive ATP synthesis. The 3 largest subunits forming the catalytic core are encoded by mitochondrial DNA, while the other smaller subunits, including COX IV, are nuclear-encoded. Research studies have shown that deficiency in COX activity correlates with a number of human diseases (4). The COX IV antibody can be used effectively as a mitochondrial loading control in cell-based research assays.

  1. Ostermeier, C. et al. (1996) Curr Opin Struct Biol 6, 460-6.
  2. Capaldi, R.A. et al. (1983) Biochim Biophys Acta 726, 135-48.
  3. Kadenbach, B. et al. (2000) Free Radic Biol Med 29, 211-21.
  4. Barrientos, A. et al. (2002) Gene 286, 53-63.
Entrez-Gene Id
1327
Swiss-Prot Acc.
P13073
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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Important Ordering Details

Custom Ordering Details: This product is assembled upon order. Please allow up to three weeks for your product to be processed.