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Product Description

This peptide is used to block Cyclin D1 (92G2) Rabbit mAb #2978 reactivity.


Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Cyclin D1 (92G2) Rabbit mAb #2978 by dot blot.

Product Usage Information

Use as a blocking reagent to evaluate the specificity of antibody reactivity in dot blot protocols.


Storage: Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. 1% DMSO. Store at –20°C.

Activity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip/Kip or INK family of proteins (1). The inactive ternary complex of cyclin D/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to affect progression through the restriction point and Rb-dependent entry into S-phase (2). The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (3). Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of protein expression and phosphorylation-dependent degradation (4).


1.  Hirai, H. et al. (1995) Mol. Cell. Biol. 15, 2672-2681.

2.  Sherr, C.J. (1996) Science 274, 1672-7.

3.  Lukas, J. et al. (1996) Mol. Cell. Biol. 16, 6917-6925.

4.  Diehl, J.A. et al. (1997) Genes Dev 11, 957-72.


Entrez-Gene Id 595
Swiss-Prot Acc. P24385


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

1044
Cyclin D1 Blocking Peptide