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Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Cytochrome c (136F3) Rabbit mAb (#4280) in the presence of control peptide (left) or Cytochrome c Blocking Peptide (right).

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Product Description

This peptide is used to block Cytochrome c (136F3) Rabbit mAb #4280 reactivity.


Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Cytochrome c (136F3) Rabbit mAb #4280 by dot blot.

Product Usage Information

Use as a blocking reagent to evaluate the specificity of antibody reactivity in dot blot protocols.


Storage: Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. 1% DMSO. Store at –20°C.

Cytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to mitochondrial intermembrane space (1). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with procaspase-9 (47 kDa)/Apaf 1. This complex processes caspase-9 from inactive proenzyme to its active form (2). This event further triggers caspase-3 activation and eventually leads to apoptosis (3).


1.  Schagger H.H. et al. (2002) Biochem. Biophys. Acta. 1555, 154-159.

2.  Li, P. et al. (1997) Cell 91, 479-89.

3.  Liu, X. et al. (1996) Cell 86, 147-57.


Entrez-Gene Id 54205
Swiss-Prot Acc. P99999


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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Cytochrome c Blocking Peptide