Upstream / Downstream
Explore pathways related to this product.
Important Ordering DetailsCustom Ordering Details: This product is assembled upon order. Please allow up to three weeks for your product to be processed.
Pricing & Additional Information
To learn more about our Blocking Peptides, including pricing, please answer a few questions.
Find answers on our FAQs page.
- Additional protein information
- Analytical tools
DARPP-32 Blocking Peptide #1081
Gallery: DARPP-32 Blocking Peptide #1081
This peptide is used to block DARPP-32 (19A3) Rabbit mAb #2306 reactivity in dot blot protocols.
The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks DARPP-32 (19A3) Rabbit mAb #2306 signal in dot blot.
Use as a blocking reagent to evaluate the specificity of antibody reactivity in dot blot protocols.Storage: Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. 1% DMSO. Store at –20°C.
DARPP-32 (dopamine and cyclic AMP-regulated phosphoprotein, relative molecular mass 32,000) is a cytosolic protein highly enriched in medium-sized spiny neurons of the neostriatum (1). It is a bifunctional signaling molecule that controls serine/threonine kinase and serine/threonine phosphatase activity (2). Dopamine stimulates phosphorylation of DARPP-32 through D1 receptors and activation of PKA. PKA phosphorylation of DARPP-32 at Thr34 converts it into an inhibitor of protein phosphatase 1 (1). DARPP-32 is converted into an inhibitor of PKA when phosphorylated at Thr75 by cyclin-dependent kinase 5 (CDK5) (2). Mice containing a targeted deletion of the DARPP-32 gene exhibit an altered biochemical, electrophysiological, and behavioral phenotype (3).
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.