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Western blot analysis of extracts from HeLa, COS, and NIH/3T3 cells using GSK-3β (27C10) Rabbit mAb #9315 (left) or the same antibody pre-incubated in the presence of GSK-3β Blocking Peptide (right).

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Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using GSK-3β (27C10) Rabbit mAb #9315 in the presence of control peptide (left) or GSK-3β Blocking Peptide (right).

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Product Description

This peptide is used specifically to block GSK-3β (27C10) Rabbit mAb #9315 reactivity.


Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks GSK-3beta (27C10) Rabbit mAb #9315 by dot blot.

Product Usage Information

Use as a blocking reagent to evaluate the specificity of antibody reactivity in dot blot protocols.


Storage: Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. 1% DMSO. Store at –20°C.

Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).


1.  Welsh, G.I. et al. (1996) Trends Cell Biol 6, 274-9.

2.  Srivastava, A.K. and Pandey, S.K. (1998) Mol Cell Biochem 182, 135-41.

3.  Cross, D.A. et al. (1995) Nature 378, 785-9.

4.  Nusse, R. (1997) Cell 89, 321-3.

5.  Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.


Entrez-Gene Id 2932
Swiss-Prot Acc. P49841


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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GSK-3β Blocking Peptide