Supplied in SDS Sample Buffer: 62.5 mM Tris-HCl (pH 6.8 at 25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT, 0.01% w/v bromophenol blue or phenol red. Store at –20°C, or at –80°C for long-term storage.
HIF-1α/2α Control Cell Extracts (Hep G2 untreated): Total cell extracts from Hep G2 cells serve as a negative control. Supplied in SDS sample buffer.
HIF-1α/2α Control Cell Extracts (Hep G2 + CoCl2): Total cell extracts from Hep G2 cells treated with cobalt chloride (100 μM, 24 hr) serve as a positive control.
This lysate pair is produced as a control for western blotting of HIF-1α and HIF-2α proteins.
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