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9160
MEK1/2 Control Cell Extracts
Experimental Controls

MEK1/2 Control Cell Extracts #9160

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  1. WB

Western blot analysis of extracts from Hela cells, untreated or TPA-treated, using Phospho-MEK1/2 (Ser217/221) (41G9) RmAb #9154 (upper) or MEK1/2 (D1A5) Rabbit mAb #8727 (lower).

Product Usage Information

Boil for 3 minutes prior to use. Load 20 µl of phosphorylated and nonphosphorylated MEK1/2 Control Cell Extracts per lane.

Storage:

Supplied in SDS Sample Buffer: 62.5 mM Tris-HCl (pH 6.8 at 25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT, 0.01% w/v phenol red or bromophenol blue. Store at –20°C or at –80°C for long term storage.

Product Description

Nonphosporylated MEK1/2 Control Cell Extracts: Total cell extracts from HeLa cells, serum starved overnight serve as a negative control. Supplied in SDS Sample Buffer.

Phosphorylated MEK1/2 Control Cell Extracts: Total cell extracts from HeLa cells, serum starved overnight then treated with 200 nM TPA #4174 for 15 minutes to serve as a positive control. Supplied in SDS Sample Buffer.

Background

MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.

  1. Crews, C.M. et al. (1992) Science 258, 478-80.
  2. Alessi, D.R. et al. (1994) EMBO J 13, 1610-9.
  3. Rosen, L.B. et al. (1994) Neuron 12, 1207-21.
  4. Cowley, S. et al. (1994) Cell 77, 841-52.
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

To Purchase # 9160S
Product # Size Price
9160S
200 µl  (10 western blots) $ 118