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  3. Phospho-ATF-2 (Thr69/71) Blocking Peptide

Phospho-ATF-2 (Thr69/71) Blocking Peptide #1040

Inquiry Info. # 1040

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    Product Information

    Product Usage Information

    For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 µl total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the relevant product data sheet.

    For Western immunoblotting, add 10 µl of antibody and 10 µl of blocking peptide to 10 ml of antibody dilution buffer, and incubate at room

    temperature for 30 minutes before allowing to react with the blot.

    Storage

    Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. Store at –20°C.

    Product Description

    This peptide is used to block Phospho-ATF-2 (Thr69/71) Antibody #9225 reactivity.

    Quality Control

    The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Phospho-ATF-2 (Thr69/71) Antibody #9225 by immunohistochemistry and Western Blotting.

    Background

    The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways (2-4). Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2-4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4). In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2).

    Alternate Names

    Activating Transcription Factor 2; atf; ATF2; phospho-atf

    Database Links

    UniProt ID: P15336


    Entrez-Gene Id: 1386


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    For Research Use Only. Not for Use in Diagnostic Procedures.
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