Use as a blocking reagent to evaluate the specificity of antibody reactivity in dot blot and Western blot protocols. For Western immunoblotting, add 10 µl of antibody and 10 µl of blocking peptide to 10 ml of antibody dilution buffer, and incubate at room temperature for 1 hour before allowing to react with the blot.
Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. 1% DMSO. Store at –20°C.
The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Phospho-CREB (Ser133) Antibody #9191 and Phospho-CREB (Ser133) (1B6) Mouse mAb #9196 by Western Blotting and Phospho-CREB (Ser133) (87G3) Rabbit mAb #9198 by Western blotting and dot blot.
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