Use as a blocking reagent to evaluate the specificity of antibody reactivity in western blotting and dot blot protocols. For western blotting, add an equal volume of peptide as volume of antibody used in 10 ml total volume, and incubate at room temperature for 30 minutes before allowing to react with the blot. Recommended antibody dilution can be found on the Phospho-eIF2alpha (Ser51) (119A11) Rabbit mAb #3597 data sheet.
Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. 1% DMSO. Store at –20°C.
The quality of the peptide was evaluated by reverse-phase HPLC and by mass spectrometry. The peptide blocks Phospho-eIF2alpha (Ser51) (119A11) Rabbit mAb #3597 signal in both dot blot and western blotting.
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