Cell Signaling Technology Logo - Extra Large
View Featured Offers >>
  1. Products /
  2. Experimental Controls /
  3. PTPRS Phosphatase

PTPRS Phosphatase #8004

Inquiry Info. # 8004

Please see our recommended alternatives.

    Product Information

    Product Description

    Purified recombinant human PTPRS (Pro883-Asn1210) phosphatase, supplied as a GST fusion protein.
    MW (kDa) 63000

    Storage

    Enzyme is supplied in 50 mM Tris-HCl, pH 7.5; 150 mM NaCl, 0.25 mM DTT, 0.1mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol, 7 mM glutathione.
    Store at -80°C.
    Keep on ice during use.
    Avoid repeated freeze-thaw cycles.

    Quality Control

    The theoretical molecular weight of the GST-PTPRS fusion protein is 63 kDa. The purified phosphatase was quality controlled for purity using SDS-PAGE followed by Coomassie stain [Fig.1]. PTPRS phosphatase activity was determined using a DELFIA® assay [Fig.2].

    Source / Purification

    The GST-phosphatase fusion protein was produced by expressing recombinant human PTPRS (Pro883-Asn1210) (GenBank Accession No. NM_130855) with an amino-terminal GST tag in E. coli. The protein was purified by one-step affinity chromatography using glutathione-agarose.

    Background

    PTPRS (PTPσ) is a receptor protein tyrosine phosphatase (PTP) belonging to the LAR family of transmembrane PTPs. The PTPRS extracellular region is composed of Ig-like and fibronectin type III-like domains; the intracellular segment contains a catalytically active membrane proximal PTP domain and an inactive PTP domain (1). This receptor protein tyrosine phosphatase is expressed primarily in neurons and likely plays some role in nervous system development (2,3). Mice deficient for PTPRS show abnormal or delayed brain and spinal cord development (4). Dorsal root ganglion neurons from PTPRS-deficient mice exhibit a higher level of N-cadherin tyrosine phosphorylation and grow more rapidly than control cells. N-cadherin has been identified in vivo as a PTPRS substrate and may be a key component in the PTPRS-mediated inhibition of axon growth (5).

    Database Links

    UniProt ID: Q13332


    Entrez-Gene Id: 5802


    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.