Render Target: STATIC
Render Timestamp: 2024-12-05T11:05:57.317Z
Commit: 224419269841c11382c4555dbee545259bf6c379
XML generation date: 2024-09-20 06:21:44.277
Product last modified at: 2024-11-11T13:15:27.586Z
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PDP - Template Name: Cell Extracts
PDP - Template ID: *******b5396df

SAPK/JNK Control Cell Extracts #9253

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    Product Information

    Product Usage Information

    Boil for 3 minutes prior to use. Load 15 µl of phosphorylated and nonphosphorylated SAPK/JNK Control Cell Extracts per lane.

    Storage

    Supplied in SDS Sample Buffer: 62.5 mM Tris-HCl (pH 6.8 at 25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT, 0.01% w/v phenol red or bromophenol blue. Store at –20°C or at –80°C for long term storage.

    Product Description

    Nonphosphorylated SAPK/JNK Control Cell Extracts: Total cell extracts from 293 cells, serve as a negative control. Supplied in SDS Sample Buffer.

    Phosphorylated SAPK/JNK Control Cell Extracts: Total cell extracts from 293 cells, treated with 50 mJ UV light and a 30 minute recovery, serve as a positive control. Supplied in SDS Sample Buffer.

    Background

    The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member (4). There are three SAPK/JNK genes each of which undergoes alternative splicing, resulting in numerous isoforms (3). SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors (3,5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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