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TRAF1 Blocking Peptide #1066
Product Description
This peptide is used to block TRAF1 (45D3) Rabbit mAb #4715 reactivity in dot blot protocols.
Quality Control
The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks TRAF1 (45D3) Rabbit mAb #4715 signal in dot blot.
Product Usage Information
Use as a blocking reagent to evaluate the specificity of antibody reactivity in dot blot protocols.
Storage: Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. 1% DMSO. Store at –20°C.Background
TRAFs (TNF receptor-associated factors) are a family of multifunctional adaptor proteins that bind to surface receptors and recruit additional proteins to form multiprotein signaling complexes capable of promoting cellular responses (1-3). Members of the TRAF family share a common carboxy-terminal "TRAF domain", which mediates interactions with associated proteins; many also contain amino-terminal Zinc/RING finger motifs. The first TRAFs identified, TRAF1 and TRAF2, were found by virtue of their interactions with the cytoplasmic domain of TNF-receptor 2 (TNFRII) (4). The six known TRAFs (TRAF1-6) act as adaptor proteins for a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress responses.
1. Arch, R.H. et al. (1998) Genes Dev. 12, 2821-30.
2. Chung, J. Y. et al. (2002) J. Cell Sci. 115, 679-88.
3. Bradley, J.R. and Pober, J.S. (2001) Oncogene 20, 6482-91.
4. Rothe, M. et al. (1994) Cell 78, 681-92.
Data Sheets & Documentation
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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