TRAF1 Blocking Peptide #1066

This peptide is used to block TRAF1 (45D3) Rabbit mAb #4715 reactivity in immunohistochemistry protocols.

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks TRAF1 (45D3) Rabbit mAb #4715 signal in immunohistochemistry.

Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry protocols. For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 μl total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the product data sheet.

TRAFs (TNF receptor-associated factors) are a family of multifunctional adaptor proteins that bind to surface receptors and recruit additional proteins to form multiprotein signaling complexes capable of promoting cellular responses (1-3). Members of the TRAF family share a common carboxy-terminal "TRAF domain", which mediates interactions with associated proteins; many also contain amino-terminal Zinc/RING finger motifs. The first TRAFs identified, TRAF1 and TRAF2, were found by virtue of their interactions with the cytoplasmic domain of TNF-receptor 2 (TNFRII) (4). The six known TRAFs (TRAF1-6) act as adaptor proteins for a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress responses.

1.  Arch, R.H. et al. (1998) Genes Dev. 12, 2821-30.

2.  Chung, J. Y. et al. (2002) J. Cell Sci. 115, 679-88.

3.  Bradley, J.R. and Pober, J.S. (2001) Oncogene 20, 6482-91.

4.  Rothe, M. et al. (1994) Cell 78, 681-92.

• Data sheet (103KB)
• MSDS PDF