Human Essential T Cell Markers Flow Cytometry Panel #96708
Important Ordering Details
Custom Ordering Details: Product is assembled upon order. Please allow up to three business days for your product to be processed.Product Information
Protocol
Product Description
T cells are identified by expression of CD3. There are two major subsets of conventional T cells: helper T cells which express CD4, and cytotoxic T cells which express CD8. Regulatory CD4+ T cells express FoxP3. Upon antigen stimulation, T cells upregulate expression of Granzyme B, a serine protease that is secreted by these cells to mediate target cell cytotoxicity.
Product Usage Information
Gating strategy for identifying essential T cell subsets:
If a fixable viability dye was used, first gate on viable cells. Next, gate on lymphocytes based on forward scatter and side scatter. Conventional helper T cells are the CD3+CD4+ cells within the lymphocyte gate. Regulatory CD3+CD4+ T cells are positive for expression of FoxP3. Conventional cytotoxic T cells are the CD3+CD8+ cells within the lymphocyte gate. Both CD3+CD4+ and CD3+CD8+ can be positive for expression of the cytotoxic molecule Granzyme B.
Storage
All components in this kit are stable in accordance with the date printed on the outer packaging label when stored at the recommended temperature. Please refer to product labels, datasheets, or web pages for specific “Best By” dates for each individual component.
Specificity / Sensitivity
Each antibody in the Human Essential T Cell Markers Flow Cytometry Panel detects endogenous levels of its target protein. CD3 (UCHT1) Mouse mAb (PE Conjugate) detects an epitope within the extracellular domain of CD3ε protein. CD4 (RPA-T4) Mouse mAb (violetFluor™ 450 Conjugate) and CD8α (RPA-T8) Mouse mAb (PE-Cy7® Conjugate) detect epitopes within the extracellular domains. FoxP3 (D6O8C) Rabbit mAb (Alexa Fluor® 488 Conjugate) and Granzyme B (D2H2F) Rabbit mAb (Alexa Fluor® 647 Conjugate) detect epitopes within the intracellular domains.
Species Reactivity:
Human
Source / Purification
Monoclonal antibodies were purified from tissue culture supernatant via affinity chromatography. The purified antibodies were conjugated under optimal conditions, with unreacted dye removed from the preparation.
Limited Uses
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