Render Target: STATIC
Render Timestamp: 2024-10-11T09:44:30.907Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-09-18 15:12:07.967
Product last modified at: 2024-09-19T08:00:11.144Z
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PDP - Template Name: InTraSeq Conjugate
PDP - Template ID: *******e148868

CD3 (UCHT1) Mouse mAb (InTraSeq 3' Conjugate 3028) #72489

Filter:
  • SCA

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Mouse IgG1
    Application Key:
    • SCA-Single Cell Analysis 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Storage

    Supplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CD3 (UCHT1) Mouse mAb (InTraSeq™ 3' Conjugate 3028) recognizes endogenous levels of total CD3ε protein. This antibody detects an epitope within the extracellular domain of CD3ε protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor.

    Background

    When T cells encounter antigens via the T cell receptor (TCR), information about the quantity and quality of antigens is relayed to the intracellular signal transduction machinery (1). This activation process depends mainly on CD3 (Cluster of Differentiation 3), a multiunit protein complex that directly associates with the TCR. CD3 is composed of four polypeptides: ζ, γ, ε, and δ. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM) (2). Engagement of the TCR complex with foreign antigens induces tyrosine phosphorylation in the ITAM motifs and phosphorylated ITAMs function as docking sites for signaling molecules such as ZAP-70 and the p85 subunit of PI-3 kinase (3,4). TCR ligation also induces a conformational change in CD3ε, such that a proline region is exposed and then associates with the adaptor protein Nck (5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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