Render Target: STATIC
Render Timestamp: 2024-10-11T10:12:12.100Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-10-10 15:16:24.303
Product last modified at: 2024-09-26T14:15:08.462Z
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PDP - Template Name: InTraSeq Conjugate
PDP - Template ID: *******e148868

S6 Ribosomal Protein (54D2) Mouse mAb (InTraSeq 3' Conjugate 3005) #38889

Filter:
  • SCA

    Supporting Data

    REACTIVITY H M R Mk Dm
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Mouse IgG1
    Application Key:
    • SCA-Single Cell Analysis 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 
    • Dm-D. melanogaster 

    Product Information

    Storage

    Supplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    S6 Ribosomal Protein (54D2) Mouse mAb (InTraSeq™ 3' Conjugate 3005) detects endogenous levels of total S6 ribosomal protein independent of phosphorylation.

    Species Reactivity:

    Human, Mouse, Rat, Monkey, D. melanogaster

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a recombinant fusion protein corresponding to full-length human S6 ribosomal protein. The epitope corresponds to a region surrounding Asp20 of human S6 ribosomal protein.

    Background

    One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of S6 protein (4,5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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