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90 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Toll-like Receptor 4 Antibody detects endogenous levels of total TLR4 protein. Cross reactivity was not detected with other family members at physiological conditions.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding serine 681 of human TLR4. Antibodies are purified by protein A and peptide affinity chromatography.

Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-4). TLRs recognize conserved motifs found in various pathogens and mediate defense responses (5-7). Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes (4). The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll/Interleukin-1 receptor (TIR) domain (1). Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains, including myeloid differentiation factor 88 (MyD88), MyD88-adaptor-like/TIR-associated protein (MAL/TIRAP), Toll-receptor-associated activator of interferon (TRIF), and Toll-receptor-associated molecule (TRAM) (8-10). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK (8,11-14). Activation of IKK leads to the degradation of IκB, which normally maintains NF-κB in an inactive state by sequestering it in the cytoplasm.

1.  Akira, S. (2003) J Biol Chem 278, 38105-8.

2.  Beutler, B. (2004) Nature 430, 257-63.

3.  Dunne, A. and O'Neill, L.A. (2003) Sci STKE 2003, re3.

4.  Medzhitov, R. et al. (1997) Nature 388, 394-7.

5.  Schwandner, R. et al. (1999) J Biol Chem 274, 17406-9.

6.  Takeuchi, O. et al. (1999) Immunity 11, 443-51.

7.  Alexopoulou, L. et al. (2001) Nature 413, 732-8.

8.  Zhang, F.X. et al. (1999) J Biol Chem 274, 7611-4.

9.  Horng, T. et al. (2001) Nat Immunol 2, 835-41.

10.  Oshiumi, H. et al. (2003) Nat Immunol 4, 161-7.

11.  Muzio, M. et al. (1997) Science 278, 1612-5.

12.  Wesche, H. et al. (1997) Immunity 7, 837-47.

13.  Suzuki, N. et al. (2002) Nature 416, 750-6.

14.  Irie, T. et al. (2000) FEBS Lett 467, 160-4.

Entrez-Gene Id 7099
Swiss-Prot Acc. O00206

For Research Use Only. Not For Use In Diagnostic Procedures.
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Toll-like Receptor 4 Antibody