3X DYKDDDDK Tag (E2T2J) Mouse mAb (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody) #33542
- WB
- IP
- IF
Supporting Data
| REACTIVITY | All |
| SENSITIVITY | Transfected Only |
| MW (kDa) | |
| Source/Isotype | Mouse IgG1 kappa |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:200 - 1:400 |
Storage
Protocol
Specificity / Sensitivity
3X DYKDDDDK Tag (E2T2J) Mouse mAb detects exogenously expressed proteins containing the DYKDDDDK epitope tag. This antibody recognizes the DYKDDDDK peptide, which is the same epitope recognized by Sigma-Aldrich Anti-FLAG M2 antibody, fused to either the amino or carboxy terminus of targeted proteins in transfected cells. 3X DYKDDDDK Tag (E2T2J) Mouse mAb detects exogenously expressed DYKDDDDK-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated. This antibody detects several non-specific bands of unknown origin in rat cell lines.
Species Reactivity:
All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the 3X DYKDDDDK epitope.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.
The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion (1).
Limited Uses
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