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MTSS1 (P549) Antibody #4385

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Inquiry Info. # 4385

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    Product Specifications

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 95-105
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MTSS1 (P549) Antibody detects endogenous levels of total MTSS1 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Pro549 of human MTSS1. Antibodies are purified by peptide affinity chromatography.

    Background

    Metastasis suppressor 1 (MTSS1) is a multi-functional scaffold protein that was initially discovered using a differential display technique that identified proteins missing from bladder cancer cell lines (1,2). MTSS1 (also known as Missing in Metastasis or MIM) is a cytoskeletal remodeling protein that contains a C-terminal WH2 actin-binding motif (1,3). Presence of an IMD (IRSp53/MIM homology) domain allows MTSS1 to induce F-actin bundling and filopodia formation in cells (4). MTSS1 binds to and activates Rac, a protein known to promote the formation of filopodia and lamellipodia (5). The receptor tyrosine phosphatase δ (PTPRD) is associated with MTSS1 and is required for MTSS1-dependent cytoskeletal change (6,7). MTSS1 is a SHH responsive gene that can help regulate GLI-dependent transcriptional activity (8).

    Alternate Names

    DKFZp781P2223; FLJ44694; KIAA0429; metastasis suppressor 1; Metastasis suppressor protein 1; Metastasis suppressor YGL-1; MIM; MIMA; MIMB; missing in metastasis; Missing in metastasis protein; MTSS I-BAR domain containing 1; MTSS1; MTSS1, I-BAR domain containing; Protein MTSS 1

    For Research Use Only. Not for Use in Diagnostic Procedures.
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