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Cleaved Gasdermin D (Asp276) Antibody (Mouse Specific)

Cleaved Gasdermin D (Asp276) Antibody (Mouse Specific) #50928

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Inquiry Info. # 50928

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Product Specifications

REACTIVITY	M
SENSITIVITY	Endogenous
MW (kDa)	31
SOURCE	Rabbit

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

M-Mouse

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Cleaved Gasdermin D (Asp276) Antibody (Mouse Specific) recognizes endogenous levels of amino-terminal fragment of Gasdermin D protein only when cleaved at Asp276.

Species Reactivity:

Mouse

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp276 of mouse Gasdermin D protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Gasdermin D (GSDMD), a member of the gasdermin family that includes GSDMA, GSDMB, and GSDMC, has been reported to have a critical role as a downstream effector of pyroptosis (1,2). Pyroptosis is a lytic type of cell death triggered by inflammasomes, multiprotein complexes assembled in response to pathogen-associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs) that result in the activation of caspase-1 and subsequent cleavage of pro-inflammatory cytokines IL-1β and IL-18 (3). Gasdermin D was identified by two independent groups as a substrate of inflammatory caspases, caspase-1 and caspase-11/4/5, producing two fragments: GSDMD-N and GSDMD-C. Cleavage results in release of an intramolecular inhibitory interaction between the N- and C-terminal domains, allowing the N-terminal fragment GSDMD-N to initiate pyroptosis through the formation of pores on the plasma membrane (4-7).

Alternate Names

1810036L03Rik; AW558049; DF5; DF5L; Dfn; Dfna5l; gasdermin D; gasdermin domain containing 1; Gasdermin domain-containing protein 1; Gasdermin-D; Gasdermin-D, C-terminal; Gasdermin-D, N-terminal; gasderminD-1; Gsdmd; GsdmD-1; GSDMD-CT; GSDMD-NT; Gsdmdc; Gsdmdc1; M2-4

Database Links

UniProt ID: Q9D8T2

Entrez-Gene Id: 69146

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