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  3. α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb #19245

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  • WB
  • IP
  • IHC
  • IF

    Supporting Data

    REACTIVITY H M R Hm Mk
    SENSITIVITY Endogenous
    MW (kDa) 42
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Hm-Hamster 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:10 - 1:50
    Immunoprecipitation 1:100
    IHC Leica Bond 1:100 - 1:400
    Immunohistochemistry (Paraffin) 1:320 - 1:1280
    Immunofluorescence (Frozen) 1:200 - 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #50513.

    Protocol

    Specificity / Sensitivity

    α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb recognizes endogenous levels of total α-smooth muscle protein.

    Species Reactivity:

    Human, Mouse, Rat, Hamster, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human α-smooth muscle actin protein.

    Background

    Actin proteins are major components of the eukaryotic cytoskeleton. At least six vertebrate actin isoforms have been identified. The cytoplasmic β- and γ-actin proteins are referred to as “non-muscle” actin proteins as they are predominantly expressed in non-muscle cells where they control cell structure and motility (1). The α-cardiac and α-skeletal actin proteins are expressed in striated cardiac and skeletal muscles, respectively. The smooth muscle α-actin and γ-actin proteins are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. The α-smooth muscle actin (ACTA2) is also known as aortic smooth muscle actin. These actin isoforms regulate the contractile potential of muscle cells (1).

    Alternate Names

    autostainer; Bond; Leica; Rx

    Database Links

    UniProt ID: P62736


    Entrez-Gene Id: 59


    Limited Uses

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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