AGPS (F1N3Q) Rabbit mAb #24043

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Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	78
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

AGPS (F1N3Q) Rabbit mAb recognizes endogenous levels of total AGPS protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu120 of human AGPS protein.

Background

Genome-wide CRISPR-Cas9 suppressor screens show that peroxisomes contribute to ferroptosis in human renal and ovarian carcinoma cells by synthesizing polyunsaturated ether phospholipids (PUFA-ePLs). PUFA-ePLs serve as substrates for lipid peroxidation. Genes identified in the screens include those for alkylglycerone phosphate synthase (AGPS) and fatty acyl-CoA reductase 1 (FAR1), two peroxisomal enzymes catalyzing ether lipid biosynthesis. Studies suggest that AGPS and FAR1 contribute to ferroptosis sensitivity (1,2). In addition, research studies show that AGPS suppresses prostate cancer cell proliferation by enhancing ferroptosis (3).

Database Links

UniProt ID: O00116

Entrez-Gene Id: 8540

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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