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  1. Products /
  2. Primary Antibodies /
  3. AIRE (F5F8X) Rabbit mAb
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Recombinant Flag
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

AIRE (F5F8X) Rabbit mAb #85045

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  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 70-80
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    AIRE (F5F8X) Rabbit mAb recognizes endogenous levels of total AIRE protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly240 of human AIRE protein.

    Background

    Autoimmune disease is a significant health concern resulting from a breakdown in normal immune self-tolerance. Immune self-tolerance is regulated by a complex system that includes key cell types, regulatory molecules, cytokines, and intricate immune cell selection processes (1-3). The autoimmune regulator (AIRE) is a transcription factor first discovered in autoimmune disease patients with rare genetic mutations (4-6). AIRE has four distinct domains: a caspase recruitment domain (CARD), the SAND domain, and plant homeodomains 1 and 2 (PHD1 and PHD2) (2,3). AIRE plays a vital role in promoting self-tolerance in the thymus by regulating expression of common tissue-restricted peripheral self-antigens called tissue-restricted antigens (TSAs) (3). AIRE is primarily expressed by medullary thymic epithelial cells (mTECs), and coordinates expression of tissue-restricted proteins that are presented on major histocompatibility complex class I and II (MHC-I, MHC-II) and to developing T cells in the thymus (2,3). AIRE facilitates self-tolerance in other ways, such as regulating mTEC differentiation, controlling thymic dendritic cell accumulation, and developing regulatory T cells via XCL1 expression (3). AIRE regulates the production of CCR4 and CCR7 binding cytokines in mTECs and can alter thymocyte development and migration (2,3). In thymic B cells, AIRE is involved in self-antigen presentation for negative selection (2,3).

    Database Links

    UniProt ID: O43918


    Entrez-Gene Id: 326


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